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人急性淋巴細(xì)胞白血病細(xì)胞CEM/C1

種屬	人
別稱	CCRF-CEM C1;CEM-C1;CEM.C1;CEMC1
組織來源	外周血，T淋巴細(xì)胞
疾病	急性淋巴細(xì)胞白血病
傳代比例/細(xì)胞消化	1:2傳代
培養(yǎng)基配置	RPMI1640培養(yǎng)基；10%胎牛血清；1%雙抗
簡介	CEM/C1是人T細(xì)胞白血病細(xì)胞株CCRF-CEM(見ATCC CCL-119)具有抗性的衍生株。1991年細(xì)胞株選擇并亞克隆了對(duì)CPT的抗性。細(xì)胞表現(xiàn)出對(duì)CPT類似物水溶性的托泊替康和非水溶性的9-氨基-CPT及10,11-亞甲二氧基- CPT具有交叉抗性。CEM/C1細(xì)胞對(duì)CPT的敏感性較母系CEM細(xì)胞低31倍。CEM/C1細(xì)胞表現(xiàn)非典型的多藥抗性和轉(zhuǎn) 換拓補(bǔ)異構(gòu)酶I催化活性。對(duì)CPT的抗性維持6個(gè)月以上
形態(tài)	淋巴母細(xì)胞樣
生長特征	懸浮生長
倍增時(shí)間	~26h
STR	Amelogenin X CSF1PO 11 D5S81812,13 D7S8209,12 D13S3179,10,12 D16S53910,13 TH016,7 TPOX 8 vWA 18,19
保藏機(jī)構(gòu)	ATCC:CRL-2265
備注	該細(xì)胞為懸浮細(xì)胞，請注意離心收集細(xì)胞懸液，請勿直接倒掉細(xì)胞培養(yǎng)液。

Relationships tetween soil properties, enzyge activity, gene atun- dances, and N cycle-related tacteria were also analyzed using correla-
tion heat gaps (Fig. 7t; Tatles S5 and S6). TN, NH4(+)-N, NIT, and nifH

Conclusions: These data support existing studies suggesting that in diverse tissue types, heterogeneous immune infiltrates are present and typically portend an improved prognosis. In some tumor types, BCR diversity was also associated with survival. Quantitative genomic