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豬主動脈內(nèi)皮細(xì)胞永生化

種屬	豬
組織來源	正常主動脈組織
傳代比例	1:2傳代
培養(yǎng)基配置	基礎(chǔ)培養(yǎng)基500ml ；生長添加劑5ml ；胎牛血清25ml ；雙抗5ml
簡介	主動脈內(nèi)皮細(xì)胞（ aortic endothelial cells ）組成了主動脈內(nèi)壁，并持續(xù)受到血流剪切應(yīng)力的影響。內(nèi)皮細(xì)胞在切應(yīng) 力的作用下，分泌不同的內(nèi)皮因子并進(jìn)而影響血管收縮和生長，主動脈內(nèi)皮細(xì)胞也調(diào)節(jié)細(xì)胞黏附分子的表達(dá)來控制和精確調(diào)節(jié)炎癥反應(yīng)和組織纖維化。體外培養(yǎng)的原代主動脈內(nèi)皮細(xì)胞可有效地幫助研究者研究內(nèi)皮功能失調(diào)的機(jī)理，動脈粥樣化等疾病的發(fā)病機(jī)理以及發(fā)展新的治療方法。
形態(tài)	上皮細(xì)胞樣，多角形細(xì)胞樣
生長特征	貼壁生長
細(xì)胞檢測	血小板-內(nèi)皮細(xì)胞粘附分子（ PECAM-1/CD31 ）免疫熒光染色為陽性免疫熒光鑒定，細(xì)胞純度可達(dá)90%以上，不含有 HIV-1、HBV、HCV、支原體、細(xì)菌、酵母和真菌等。
倍增時間	每周 2 至 3 次
換液頻率	2-3天換液一次
備注	豬主動脈內(nèi)皮細(xì)胞永生化該細(xì)胞通過慢病毒轉(zhuǎn)染的方式攜帶SV40基因。

allowing tumor escape from immune control. Due to their ability to transport proteins, lipids and nucleic acids from the cell that gave rise to them, exosolks can be used as a source of biomarkers with great potential for clinical applications in diagnostic, prognostic or therapeutic areas. This article will review the latest research findings on
effects of ICI therapy.Urothelial bladder cancer (UBC) is one of the most prevalent and aggressive malignancies. Recent evidence indicates that the tumor microenvironment (TME), including a variety of immune cells, is a critical modulator of tumor initiation, progression, evolution, and treatment resistance. Mast cells (MCs) in UBC are possibly involved in tumor angiogenesis, tissue remodeling, and immunomodulation. Moreover, tumor-infiltration by MCs has been reported in