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RG001060-anti-FLAG Tag Rabbit Polyclonal antibody (兔多克隆抗體)
  • RG001060-anti-FLAG Tag Rabbit Polyclonal antibody (兔多克隆抗體)

貨物所在地:北京北京市

地: 北京通州

更新時間:2025-02-13 21:00:07

瀏覽次數(shù):717

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anti-FLAG Tag Rabbit Polyclonal antibody (兔多克隆抗體)在Western Blotting中使用內(nèi)參其實就是在WB過程中的另外用內(nèi)參對應(yīng)的抗體檢測內(nèi)參,這樣在檢測目的產(chǎn)物的同時可以檢測內(nèi)參的表達(dá),由于內(nèi)參在各組織和細(xì)胞中的表達(dá)相對恒定,借助檢測每個樣品內(nèi)參的量就可以用于校正上樣誤差,這樣半定量的結(jié)果才更為可信。

英文名稱      anti-FLAG Tag Rabbit Polyclonal antibody

應(yīng)用             WB 1:2500 IF 1:100 IP 1:100 Optimal dilutions should be determined by the end user.

規(guī)  格           100ul

濃  度           1mg/ml

保  存           Store at -20°C. Do not aliquot the antibody.

儲存液          PBS, pH 7.4, containing 0.02% sodium azide as Pretive and 50% Glycerol

純化方法      affinity purified by Protein A

亞  型           IgG

性  狀           Liquid

克隆類型      polyclonal

宿  主           Rabbit

The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.

說明書

Anti- Flag Tag Rabbit Polyclonal Antibody

Catalog No. RG001060

Size 100μl

Storage: Store at -20°C. Do not aliquot the antibody.

The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to

interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively

as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to

separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can

also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many

different assays that require recognition by an antibody. If there is no antibody against the studied protein,

adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag

sequence.

Molecular Weight: N/A

Application: WB 1:2500 IF 1:100 IP 1:100 Optimal dilutions should be determined by the end user.

Specificity: The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag

fusion proteins.

Form: Liquid , 1mg/ml

Storage buffer: PBS, pH 7.4, containing 0.02% sodium azide as Pretive and 50% Glycerol

Relative Products

M1001010 Anti- HA Tag Mouse Monoclonal Antibody(2C7)

M1001020 Anti-His Tag Mouse Monoclonal Antibody(3C5)

M1001030 Anti-GFP Tag Mouse Monoclonal Antibody(2H7)

M1001040 Anti-GST Tag Mouse Monoclonal Antibody(2C7)

M1001050 Anti- cMyc Tag Mouse Monoclonal Antibody(2C5)

M1001060 Anti- Flag Tag Mouse Monoclonal Antibody(1B3)

RG001010 Anti- HA Tag Rabbit Polyclonal Antibody

RG001020 Anti- His Tag Rabbit Polyclonal Antibody

RG001030 Anti- GFP Tag Rabbit Polyclonal Antibody

RG001040 Anti- GST Tag Rabbit Polyclonal Antibody

RG001050 Anti- cMyc Tag Rabbit Polyclonal Antibody

RG001060 Anti- Flag Tag Rabbit Polyclonal Antibody

Solarbio


Beijing Solarbio Science & Technology Co., Ltd

Http://www.solarbio。。com
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